rabbit polyclonal anti p63 antibody  (Elabscience Biotechnology)

Journal: Scientific reports

Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.

doi: 10.1038/s41598-025-96919-2

Figure Lengend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

Article Snippet: After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control

Journal: Journal of Veterinary and Animal Sciences

Article Title: Pathology and evaluation of immunohistochemical expression of tumour protein 63 (p63) in corneal squamous cell carcinoma in a dog

doi: 10.51966/jvas.2025.56.1.196-199

Figure Lengend Snippet: Fig. 5. Squamous cell carcinoma-p63 expression –Strong (DAB,

Article Snippet: The sections were tested with rabbit polyclonal anti-p63 antibody (1:50, Elabscience Biotechnology, USA) and was incubated overnight.

Techniques: Expressing

Journal: Heliyon

Article Title: Electrospun poly( l -lactide- co - dl -lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

doi: 10.1016/j.heliyon.2024.e30970

Figure Lengend Snippet: Cell appearance as found by live cell and immunofluorescence imaging. (A) Live cell staining (CytoCalcein Violet 450, blue). Plasma membranes were visualized using CellMask™ Plasma Membrane Deep Red staining (red). (B) Immunocytochemistry for both stemness marker p63α (green) and epithelial cell marker keratin 7 (red). Cell nuclei were counterstained with DAPI (blue). Note the smaller nuclei and cells in fibrin gel culture compared to PDLLA membrane culture.

Article Snippet: Incubation with anti-p63α rabbit polyclonal antibody (#4892S, Cell Signalling, Danvers, MA, USA) and anti-keratin 7 mouse monoclonal antibody (#OV-TL-12/30, Zeta Corporation, Arcadia, CA, USA) at a dilution of 1/250 was carried out at 4 °C overnight.

Techniques: Immunofluorescence, Imaging, Staining, Clinical Proteomics, Membrane, Immunocytochemistry, Marker